All offices and laboratories of program faculty are on the University of Georgia (UGA) main campus within walking distance of one another. A brief description of the facilities available for training is below.
The BFF provides animal cell culture, fermentation, high-containment services, molecular biology, monoclonal antibodies, peptide synthesis, process development, and purification to UGA, other academic researchers and industry. The state-of-the-art facility includes fermentation equipment from 750mL to 750L; downstream processing equipment including homogenization (12k psi), TFF (10 m2) & hollow fiber (up to 12 m2); preparative & analytical HPLC; low pressure chromatography (up to 3 L/min.); an array of chromatography equipment for projects (up to 25L packed column); and shelf lyophilizers (purified proteins only).
The BIRC is a multi-imaging research suite designed to provide a full range of biological tissue imaging technologies to multiple biomedical investigators, their students, and other researchers. The facility houses a GE 16-channel fixed-site Signa HDx 3.0 Tesla Magnetic Resonance Imaging (MRI) magnet with MRI, fMRI, MRS, MRA, DWI and DTI capabilities; a CTF Omega Whole-cortex magnetoencephalography system with 143 channel MEG and 32 channel EEG, all enclosed in a state-of-the-art magnetically shielded room; an EGI Geodesic System 200 Electroencephalography system with 256-channel head capabilities; and a 7T Varian Magnex 7- Tesla 210mm horizontal bore MRI/MRS system with 44 gauss/cm gradient field strength, multiple volume coils for imaging mice and rats. Advanced MRI and MRS techniques are available for non-invasive morphological, physiological, and biochemical and spectroscopic measurement. birc.uga.edu.
The BMC is an optical microscopy facility established in 2012 to support the biological and biomedical research of the UGA research community. The BMC provides access to several state-of-the-art microscopes that are uniquely suited for live cell imaging of various model organisms over time, 2-D and 3-D conventional, deconvolution, high-content and confocal imaging of fixed and live cell, tissue and organ samples, and super resolution imaging of subcellular structures. The Director of the Core facility, Dr. M.K. Kandasamy, is a highly experienced cell biologist with more than thirty years of microscopy related research experience, and provides users training and assistance in acquiring and analyzing data, and help in designing and interpreting microscopyrelated experiments.
Located at UGA’s Complex Carbohydrate Research Center, this facility is used to determine molecular structures of carbohydrates and proteins and to investigate the structural and dynamical basis of protein-carbohydrate interactions. It consists of seven high-field Agilent spectrometers (a 300 MHz, 500 MHz (wide bore), 600 MHz, 800 MHz (NMR regional facility equipment with flow cell option), and 900 MHz (Southeast Collaboratory highfield biomolecular) spectrometers. Standard laboratory facilities and supplies are available for sample preparation. SUN and PC workstations running Felix (MSI), VNMR and NMRPipe are available for data processing. Data storage to CD, DVD or 4mm DAT tape is available on site. ccrc.uga.edu/~ccrcnmr/nmrfacility.html.
The Complex Carbohydrate Research Center offers Analytic Services to universities, research institutes and biotechnology and pharmaceutical companies interested in analysis, structural elucidation and validation of polysaccharides, glycoproteins, and glycolipids. The Analytical Service Team offers both routine services and in-depth full structural characterization of samples derived from plant, animal or bacteria, or produced through cell culture.
The Comparative Pathology Laboratory is dedicated to providing expert diagnostic and research pathology (biopsy and necropsy) services to investigators using laboratory animals in their research projects. It offers diagnostic pathology research services in many areas of expertise—including avian pathology, laboratory animal comparative pathology, infectious diseases, wildlife and zoological pathology. The laboratory is directed by a Ph.D./DVM diplomate of the American College of Veterinary Pathologists.
The Flow Cytometry Core is a full service flow assisted cell-sorting (FACS) facility that provides access to state-of-the-art flow cytometry equipment. The core includes a Becton Dickinson FACSCalibur with 2 laser excitation (488 and 634) and 4 emission detectors, a Beckman Coulter CytoFLEX with 4 laser excitation (405, 488, 561 and 634) and 11 emission detectors, two Beckman Coulter CyAns with 3 laser excitation (405, 488 and 634) and 9 emission detectors, and a Beckman Coulter MoFlo XDP cell sorter with 4 laser excitation (405, 488, 561 and 634) and 11 emission detectors. The core also includes two cell sorters, a MoFlo XDP that can sort in to any plate platform and can sort up to 4 different populations simultaneously in to tubes, and a Bio-Rad S3 cell sorter with 2 laser excitations (488 and 561) and 4 emission detectors that can sort up to 2 different populations simultaneously. The core also houses a Bio-Rad Bio-Plex multiplexing Luminex bead-based instrument, and a Millipore ImageStream flow imager with 4 laser excitation (405, 488, 561, 634) and 11 emission detectors. The facility, co-located with the UGA Center for Tropical and Emerging Global Diseases, also provides expert advice and consultation for the design and analysis of flow experiments. https://ctegdcytometry.uga.edu
The Electron Microscopy Laboratory offers electron microscopy and related services to the veterinary and medical professions. A complete range of conventional preparative services are offered, allowing investigators to submit fresh or fixed tissues and receive representative study prints in digital format or from scanned negatives. Special services include direct negative staining, immuno electron microscopy, and immunocytochemistry
IThe Georgia Advanced Computing Resource Center (GACRC) provides high-performance computing hardware and network infrastructure, as well as consulting and training services in support of world-class research computing and communications resources for UGA researchers. Located in UGA’s Boyd Data Center (BDC), the GACRC has a fulltime staff of six systems administrators and scientific computing consultants specializing in Linux/UNIX system administration, storage administration, and scientific computing consultation. The primary computational resource is a 2600 compute-core Linux cluster which, in addition to conventional compute nodes, has several large memory and GPU specific nodes. High-performance storage for the Linux cluster is provided for users’ home directories and temporary scratch space. Slower storage resources are available for long-term archival needs. Expandable access to Internet2, Southern Light Rail and National Lambda Rail networks are also available. The GACRC provides better than 99.99% uptime to its users of computing and storage resources and serves over 150 principal investigators and over 450 total users.
Georgia Electron Microscopy (GEM) offers services within and outside UGA to biological, biomedical sciences, plant biology, geology, chemistry, textiles, archaeology, agriculture, physics, and nanotechnology/materials analysis. GEM provides a unique medley of state-of-the-art equipment, a highly competent technical staff, and a broad education mission. Instrumentation includes a Leica SP5 confocal/multi-photon microscope, FEI Technai 20 TEM, SPI module sputter coater, Tousimis critical point dryer, ultramicrotomes, Zeiss 1450 EP SEM, Leica SP2 spectral confocal microscope, and light microscopes.
The Georgia Genomics Facility (GGGF) is a core sequencing and genotyping laboratory whose sequencing services include single tube and 96-well plate based capillary (Sanger) sequencing, as well as Next Generation DNA Sequencing (Illumina, 454, Ion Torrent). Genotyping services include capillary fragment analyses (e.g., microsatellite DNA) and multiple platforms for SNP genotyping (BeadXpress, Open Array, and high resolution melt curves via realtime PCR). Real-time PCR services for gene expression and SNP genotyping studies are available using a Roche 480 Lightcycler.
The Georgia X-Ray Crystallography Center (GXRCC) is one of the most advanced and enabling academic Xray crystallography laboratories in the world in terms of both availability of state-of-the-art equipment and new technology development. In collaboration with the Southeast Regional Collaborative Access Team (SER-CAT), GXRCC has facilitated in the construction of two synchrotron beamlines at the Advance Photon Source (APS), Argonne National Laboratory. The GXRCC administers the SER-CAT program and operates the SER-CAT facility at the APS. GXRCC is also the home research site for the Southeast Collaboratory for Structural Genomics (SECSG), one of nine NIH-funded PSI pilot centers.
The Integrated Bioscience and Nanotechnology Cleanroom includes a 2,200-square-foot (Phase I) Class 100/1,000 multidisciplinary, nanotechnology-focused fabrication, characterization, and manipulation facility and a 1,000-square-foot (Phase II) Class 10,000 bio-cleanroom part. This campus-wide shared facility can also be accessed by users outside UGA.
Proteomic and Mass Spectrometry Facility services include Electrospray (ESI), GC-MS, LC-MS, FT-MS, MALDI, high resolution (FT-MS) EI direct probe, and high resolution (FT-MS) ESI. For example, the facility is equipped with an ABI 4700 Proteomics Analyzer (MALDI/TOF-TOF) mass spectrometer for performing matrix assisted laser desorption ionization (MALDI) TOF analysis and is also capable of doing MALDI M-MS analysis. This instrument is made for high-performance analysis of tryptic digests and looking at peptide fingerprints (trypsin digests of proteins).